Read e-book online Affinity Labeling: Affinity Labeling PDF

By Nathan P. Kaplan, William B. Jakoby, Meir Wilchek

The seriously acclaimed laboratory general, equipment in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. when you consider that 1955, each one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct this present day - actually an important booklet for researchers in all fields of lifestyles sciences.

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K D. H. Buttlaire and M. Cohn, J. Biol. Chem. 249, 5733 (1974). K. A. Koetfler and G. P. Hess, Biochemistry 18, 5345 (1974). K. A. Koehler and G. E. Lienhard, Biochemistry 10, 2477 (1971). R. N. Lindquist and C. Terry, Arch. Biochem. Biophys. 160, 135 (1974). ° J. O. Westerik and R. Wolfenden, J. Biol. Chem. 247, 8195 (1971). P R. C. Thompson, Biochemistry 12, 47 (1973). J. O. Westerik a n d R . Wolfenden, J. Biol. Chem. 249, 6351 (1974). r j. D. Findlater and B. A. Orsi, FEBS Left. $6, 109 (1973) ° J.

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Glover, and J. M. Prescott, Arch. Biochem. Biophys. 165, 739 (1974). [3] M e c h a n i s m - B a s e d I r r e v e r s i b l e Enzyme Inhibitors By ROBERTR. RANDO There are two extreme forms of mechanism for specific irreversible enzyme inhibitors. One comprises the classical affinity labeling agents, which are substrate analogs containing chemically reactive functional groups. 1 The specific binding of these inhibitors to the active-site regions of the enzyme ensures a greater probability of chemical reaction with a residue within this region as opposed to nonspecific chemical reactions.

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Affinity Labeling: Affinity Labeling by Nathan P. Kaplan, William B. Jakoby, Meir Wilchek


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