By Margaret I. Tyler (auth.), Catherine Cooper, Nicolle Packer, Keith Williams (eds.)
Amino acid research is time-honored in biotechnology, biomedical, and meals research laboratories. Amino Acid research Protocols constitutes an enormous choice of those integral analytical innovations, either vintage and state of the art, of excessive application for answering particular organic questions. universal equipment contain these according to HPLC or fuel chromatography separation and research after precolumn derivatization. New options in keeping with capillary electrophoresis separation, high-performance anion trade chromatography, and mass spectrometry also are awarded. considering effects rely seriously at the caliber of the pattern, such a lot members have dedicated a bit to pattern coaching, quite to the gathering and garage of physically fluids. a brand new strategy for desalting samples sooner than hydrolysis is additionally supplied. each one process is defined in step by step aspect to make sure profitable experimental effects, and comprises priceless notes on pitfalls to prevent, and adaptations that allow the easy methods to be used with various systems.
updated and hugely useful, Amino Acid research Protocols deals analytical and medical chemists, in addition to a wide diversity of organic and biomedical investigators, a wealthy compendium of laboratory instruments for the efficient research of either universal and unusual amino acids.
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Extra info for Amino Acid Analysis Protocols
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Hydrolysis 1. Use a pipet or syringe to place a protein or peptide sample in a 6 × 50-mm glass hydrolysis tube. 2. Use a vacuum system, a stream of warm nitrogen, or a freeze dryer to remove the solvent from the sample. 3. , a Pico-Tag® vacuum vial, Waters Corporation). Add 200 μL of constant boiling HCl into the bottom of the vacuum vial. 4. Add a crystal of phenol or a drop of liquefied phenol to the bottom of the vial, and seal the vial after three alternate steps each of vacuum and nitrogen purging, finishing with a vacuum step.
2. Place ProSorb device in an appropriate test tube rack. 3. Wet the PVDF membrane in the reservoir with 10 μL of methanol (see Note 12). 4. Add sample to reservoir (100 to 400 μL volume), and allow the sample to pass through the membrane (see Note 13 and 14). 5. Ensure that the sample reservoir has intimate contact with the absorbent and fluid transfer begins. 6. Add additional sample aliquots, if necessary (change absorbent after each 750 μL). 7. 1% TFA two times. 8. Remove sample reservoir and air-dry the PVDF membrane.
Amino Acid Analysis Protocols by Margaret I. Tyler (auth.), Catherine Cooper, Nicolle Packer, Keith Williams (eds.)